Molecular Assays for Detection of Human Metapneumovirus
نویسندگان
چکیده
منابع مشابه
Molecular assays for detection of human metapneumovirus.
The recent description of the respiratory pathogen human metapneumovirus (hMPV) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory tract infections. We describe two novel approaches to the detection of viral RNA by use of reverse transcriptase PCR (RT-PCR). The PCR products were identified after capture onto a solid-phase mediu...
متن کاملMolecular Epidemiology of Human Metapneumovirus
1Department of Biological Science, Okinawa Prefectural Institute of Health and Environment, Okinawa 901-1202; 2Awase Daiichi Clinic, Okinawa 904-2172; 3Aozora Pediatric Clinic, Okinawa 901-1302; 4Gushi Kodomo Clinic, Okinawa 901-0244; 5Gunma Prefectural Institute of Public Health and Environmental Sciences, Gunma 371-0052; and 6Infectious Disease Surveillance Center, National Institute of Infec...
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The human metapneumovirus (hMPV) is a new member of the Paramyxoviridae family associated with acute respiratory tract infections in humans. The objective of this study was to compare the sensitivity of real-time RT-PCR assays performed in a LightCycler instrument and designed to amplify the viral nucleoprotein (N), matrix (M), fusion (F), phosphoprotein (P), and polymerase (L) genes. In a firs...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.1.100-105.2003